Protective Effects of Propolis Against the Amitraz Hepatotoxicity in Mice

The present study aimed to study the protective effects of honeybee propolis against the amitraz hepatotoxicity in mice. Forty-eight Swiss albino male mice of 8 weeks of age, 22 to 25 g body weight were divided into four groups. The 1 was control, the 2 was treated orally with 150 mg kg propolis extract, the 3 was treated with 160 mg kg amitraz and the 4 one had 160 mg kg amitraz + 150 mg kg propolis extract. These daily treatments lasted for 8 weeks and laboratory assays were measured weekly. Results, after mice sacrificed, histopathology and immunohistology tests were carried out. The obtained results revealed that amitraz had affected liver biochemicals concentrations, whereas propolis led to a significant decrease in these levels in treated group. But, hepatocytes of mice treated with amitraz + propolis demonstrated positive stained nuclei, by using Ki67 immunostaining, less than those of amitraz treated only. The study suggests that propolis ameliorated the recovery of hepatotoxicity of amitraz in the tested mice.